Kinetic analysis of regulated myosin ATPase activity using single and limited turnover assays.
نویسندگان
چکیده
Myosin from molluscan adductor muscle is regulated directly by Ca2+ binding. In the absence of Ca2+ the ATPase activity is greatly inhibited. We review the application of transient kinetic methods to this system and show how they can be simple to perform and less ambiguous than steady-state methods.
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عنوان ژورنال:
- Journal of cell science. Supplement
دوره 14 شماره
صفحات -
تاریخ انتشار 1991